![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K4750003:Design
Designed by: Anne-Katrin Wilbrink, Johannes Radde Group: iGEM23_TU-Dresden (2023-10-10)
SP+T8 (tpSil3)_eGFP_FAST-PETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 898
Illegal PstI site found at 169 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 898
Illegal PstI site found at 169 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 898
Illegal BglII site found at 143
Illegal XhoI site found at 1693 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 898
Illegal PstI site found at 169 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 898
Illegal PstI site found at 169 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Due to assembly, there is a 2 AA long linker between T8 and eGFP, and a 4 AA long linker between eGFP and FAST-PETase
Source
BBa_K4750001, BBa_K1123017, BBa_K4750002