Coding

Part:BBa_K4750003:Design

Designed by: Anne-Katrin Wilbrink, Johannes Radde   Group: iGEM23_TU-Dresden   (2023-10-10)


SP+T8 (tpSil3)_eGFP_FAST-PETase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 898
    Illegal PstI site found at 169
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 898
    Illegal PstI site found at 169
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 898
    Illegal BglII site found at 143
    Illegal XhoI site found at 1693
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 898
    Illegal PstI site found at 169
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 898
    Illegal PstI site found at 169
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to assembly, there is a 2 AA long linker between T8 and eGFP, and a 4 AA long linker between eGFP and FAST-PETase


Source

BBa_K4750001, BBa_K1123017, BBa_K4750002

References